Overview
With our Clariostar - Well Plate integration, users can now connect their well plate layouts within Scispot with their Clariostar Microplate Reader. Data measured by the reader can then be automatically sent back to Scispot and sync with the corresponding well plate within the platform! This allows users to keep all of their most important information in the same place, while still being able to make use of their favourite instruments.
TL;DR
Here is the official guide from Clariostar that highlights the details regarding importing and exporting data to and from Clariostar and Scispot
Part 1: Exporting Multi Well Plates to Clariostar Microplate Reader
Step 1: Create a Multi Well Plate
Whether through a protocol template with an embedded manifest template, or through a manifest on its own, create a new manifest for testing with Clariostar
Add your sample data stored in a Labsheet and layout the samples in the manifest as desired
Step 2: Export the Multi Well Plate in Clariostar compatible format
In the top right hand corner of your manifest, under the well plate tab, select Export
Select the desired instrument from the Instruments dropdown
Based on the instrument selection, compatible file types will appear in the Supported File Type dropdown - select the desired file type
Once this information is complete, select Export to download a Clariostar compatible file of the created multi well plate
Step 3: Import the plate into Clariostar
Within the Clariostar control software, navigate to the Start Measurement screen and select a multi well plate that is the same size as the one created within Scispot
Select the Samples IDs / Dilution Factors tab to open the well plate table for the new test
Select Import IDs in the bottom right hand side of the screen and in the resulting file selector, choose the downloaded multi well plate file from Step 2
Step 4: Run your measurement!
The control software on your plate reader will now populate with sample ID values, creating a mirror of your Scispot multi well plate on your Clariostar and completing the setup of your well plate for testing
Ensure that you have setup your Clariostar to save data in ASCII format - help in setting this up can be found here
Part 2: Syncing Results and Multi Well Plates
For an automated live syncing solution of data from the Clariostar plate reader, check out our article on using a Scispot GLUE!
Step 1: Upload data
Within the manifest on Scispot used to create the multi well plate used above, navigate to the Analysis tab
In the top right hand corner of the screen, select Import Columns
Under Source Type, select the Clariostar plate reader instrument
Select Upload file and select the ASCII file from your plate reader corresponding to your test run
Select Import to automatically get the columns of data from your test
Step 2: Import data
Select the columns from the file to be added to the Analysis sheet
Select Add selected columns to add them to the Analysis sheet
Step 3: Analyze data
Now with the data imported into the Analysis sheet, formula columns can be created to help analyze and visualize the resulting data
Data from other existing labsheets can also be imported into the analysis through the Import Columns -> Labsheet flow
Once you bring in your data from Clariostar to your well-plates, you can add calculated columns to it as well, such as calculation for protein concentration
Add a column using the + icon, name it Protein Conc 1:10 (562nm)
Apply your formula. For instance, if you are subtracting a constant from the absorbance, and then dividing it with the gradient
Part 3: Syncing Analysis with Labsheets
Labsheets are your single source of truth so once all the analysis is done, you can bring that data back to your Labsheets